Resveratrol is a phytoalexin, which has functions of anti-free radicals and reducing incidence of coronary heart disease. Recent studies have found that resveratrol can inhibit the three main stages of cancer initiation, promotion and expansion. It is a promising anticancer drug. Therefore, the analysis and research on resveratrol has important theoretical and application value.
Resveratrol under natural conditions in order to free state and glucosidase are two forms of existence. Judging from the chemical structure of resveratrol and its glucoside both cis and trans isomers. About resveratrol, cis, trans isomers of each other change as well as the original ingredients of resveratrol and its glycosides in a form of confirmation, but also no studies have reported.
Using HPLC analysis of resveratrol and its glucoside four monomers analysis methods, and apply this method to the analysis of the tiger battle Pieces to confirm the tiger battle in a form of resveratrol and resveratrol glucoside levels were measured. -cyclodextrin can simulate not only the body enzymes and receptors of chiral macromolecules can also serve as safe and effective inclusion material to improve the physical and chemical properties of the drug. Therefore, to study the affinity interaction between -cyclodextrin with resveratrol four kinds of monomers has important theoretical and practical significance.
Due to cis resveratrol unstable, unable to obtain pure, so the application of nuclear magnetic resonance method, UV - visible spectroscopy, fluorescence spectroscopy can not be measured was trans-resveratrol with -cyclodextrin combination of constants. Resveratrol with -cyclodextrin are neutral compounds, the use of traditional capillary electrophoresis can not be measured by their binding constants.
Charged cyclodextrin in the running buffer, Dual Cyclodextrin Systems affinity capillary electrophoresis determination of neutral compounds with neutral cyclodextrin binding constants and determination of -cyclodextrin resveratrol and its glycoside binding constants of the four monomers. The difference resveratrol four monomers and -cyclodextrin binding constants, the researchers discussed their inclusion mechanism.
Poor water solubility of resveratrol and UV prone isomerization greatly limit its application. The pharmaceutical raw materials suppliers and scientists studied the influence of three kinds of cyclodextrin on water-soluble and ultraviolet irradiation stability of resveratrol. And they also discussed about the behavior of resveratrol and three cyclodextrin inclusion. They solved the problems that resveratrol is insoluble in water and it has poor UV stability. Some exploratory works have been done for the preparation of resveratrol. It further broadens the scope of application of cyclodextrins and their derivatives.Source:http://www.cospcn.com