Paternity Test Protocol
- First, DNA is obtained from both the child and the alleged father by swabbing the mouth and isolating cells from the fluids gathered.
- The DNA from both the man and the child is cut into sections by enzymes. These sections are then amplified--or multiplied--using the PCR procedure.
- Fluorescent markers that bind to specific sequences of the DNA are applied to the sections. If the man is the father of the child, their DNA will contain similar patterns of fluorescence.
- In this method, the DNA of man and child are cut using special enzymes called restriction enzymes. The resulting fragments are spread out according to size using a technology called gel electrophoresis.
- If the gel patterns formed by the DNA fragments show a certain degree of similarity between the man and child's fingerprints, the man will be declared the father of the child.
Obtaining DNA
Amplifying DNA with PCR
Applying Fluorescent Markers
Constructing DNA Fingerprints
Comparing the DNA Fingerprints
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