Discrepancies in BRAF and EGFR Mutation Detection
Discrepancies in BRAF and EGFR Mutation Detection
Repeat DNA measurements (n=78) were performed at UCL and Asuragen using a Qubit fluorometer (Invitrogen). Qubit was selected over qPCR due to its more frequent use alongside Nanodrop. The Qubit dsDNA HS Assay Kit (Invitrogen) was used for quantitation. Assay calibration was performed using the internal controls provided. Measurements were correlated between the two sites and compared with participants' measurements.
Quantitation
Repeat DNA measurements (n=78) were performed at UCL and Asuragen using a Qubit fluorometer (Invitrogen). Qubit was selected over qPCR due to its more frequent use alongside Nanodrop. The Qubit dsDNA HS Assay Kit (Invitrogen) was used for quantitation. Assay calibration was performed using the internal controls provided. Measurements were correlated between the two sites and compared with participants' measurements.
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