How to Trace the Presence of Organic Molecules
- 1). Turn on the spectrofluorometer. Make sure the absorption cells are clean. If the cells are not completely clean, errors could occur in the spectrophotometric results. In addition, do not touch the cells with your hands, as that could leave marks or a film on the cells, which can also alter the results.
- 2). Take a dropper and fill it with your sample solution. Rinse the cells several times with the solution. Fill a pipette with the sample solution and add the solution to the cells. Make sure you don't overfill the cells.
- 3). Insert the cells into the cell holder. Make sure that the cells are in the same direction as the cell holder.
- 4). Take your pipette and fill a plastic cuvette with the sample solution. Put the plastic cuvette into the sample compartment of the spectrofluorometer. Close the spectrofluorometer.
- 5). Run the spectrofluorometer. Record both the excitation and emission maximums. The data from the spectrofluorometer during the excitation state will show a plot. The points in the plot will represent different complex samples of organic compounds. The plot will assist in distinguishing each complex sample.
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