What Is Used to Sterilize Lab Equipment?
- Sterilization is the destruction all microorganisms. For something to be sterile, it must be have no active microbes and be free of bacterial spores.
- Laboratories have a wide range of equipment, depending on their specialty. Common types include glassware, pipettes, stoppers, clamps, scalpels, syringes/needles and thermometers.
- There are two types of heat sterilization: dry and moist. Moist heat sterilization is performed in an autoclave, subjecting items to steam under pressure, at temperatures of 121 degrees Celsius for 15 to 20 minutes. Dry heat sterilization is done in an oven and requires higher heat and longer incubation times.
- Bacillus subtilis is a heat-resistant bacteria used to test the efficiency of the sterilization process. If these bacterial spores are killed, then all other microorganisms are also eliminated.
- The autoclave was invented by Charles Chamberland in 1879. Chamberland also worked with Louis Pasteur to develop one of the very first vaccines for chicken cholera in 1881.
Features
Types of Lab Equipment
Sterilization Methods
Measuring Efficiency
Fun Fact
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